Pre-clinical results with Clariscan (NC100150 Injection) : experience from different disease models

• A superparamagnetic nanoparticle (NC100150 Injection) was investigated in two different animal models; renal perfusion in pigs and tumour imaging in mice. In the pig model, qualitative first-pass perfusion maps following a bolus injection of NC100150 Injection enabled good visualisation of hypoperfused regions of the renal cortex following partial ligation of the renal artery. High temporal resolution was found to be essential to accurately capture the first passage of the contrast agent through the kidney due to the very rapid blood flow in normal renal cortex. In the tumour model (LS174T cells implanted in nude mice), NC100150 Injection was found to cause a gradual (over 60 min) signal increase on T1-w images in part of the tumours which was attributed to contrast agent leakage from the vascular space to the extravascular space in areas of increased capillary permeability. This observation is consistent with previous reports on the molecular cut-off size for vascular extraction for this tumour cell line. The specific enhancement of tumour tissue suggest potential utility of NC100150 Injection as an angiogenesis marker.

Nyckelord

Adenocarcinoma/*diagnosis

Animals

Colonic Neoplasms/*diagnosis

Contrast Media

Disease Models; Animal

Humans

Iron/*diagnostic use

Ischemia/diagnosis

Kidney/*anatomy & histology/*blood supply

Kidney Cortex/blood supply

Magnetic Resonance Imaging/*methods

Mice

Mice; Nude

Oxides/*diagnostic use

Renal Artery Obstruction/*diagnosis/physiopathology

Sensitivity and Specificity

Swine

Transplantation; Heterologous

Tumor Cells; Cultured

Publikations- och innehållstyp

vet (ämneskategori)

art (ämneskategori)