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A fibronectin-bindi...
A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction
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- Lidén, Åsa (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Karlström, Åsa (författare)
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Lannergård, Jonas (författare)
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- Kalamajski, Sebastian (författare)
- Lund University,Lunds universitet,Åke Oldbergs forskargrupp,Forskargrupper vid Lunds universitet,Åke Oldberg´s group,Lund University Research Groups
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Guss, Bengt (författare)
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- Rubin, Kristofer (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Rydén, Cecilia (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Institutionen för medicinska vetenskaper,Friman, infektion
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- Engelska.
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 340:2, s. 604-610
- Relaterad länk:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemicus was investigated as to effects on murine cell interactions with extracellular matrix proteins. FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alphaVbeta3. C2C12 cells lack collagen-binding beta1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel contraction by C2C12 cells but not adhesion of C2C12 cells to collagen. Experiments with an alphaVbeta3-inhibitor suggested that FNZN promoted contraction by a process requiring alphaVbeta3. Our data suggest that FNZN by binding to cells, collagen, and FN modulate complex adhesive processes mediated by the alphaVbeta3 integrin. Since alphaVbeta3-mediated contractile events function to counteract edema formation during inflammation, it is possible that FNZN and its secreted homologue FNE modulate edema responses in infected tissues.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Adhesins; Bacterial/*metabolism/physiology
- Animals
- Cattle
- Cell Adhesion/physiology
- Cell Line
- Collagen/*metabolism
- Extracellular Matrix Proteins/metabolism
- Gels/metabolism
- Humans
- Mice
- Myoblasts/metabolism
- Research Support; Non-U.S. Gov't
- Streptococcus equi/chemistry/*metabolism
- Transition Temperature
- MEDICINE
- MEDICIN
- infection
- adhesion
- edema
- collagen structure
- collagen-binding
- integrins
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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