Sökning: id:"swepub:oai:DiVA.org:uu-79646" >
Template convolutio...
Template convolution to enhance or detect structural features in macromolecular electron-density maps
-
- Kleywegt, GJ (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Strukturell molekylärbiologi
-
- Jones, TA (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Strukturell molekylärbiologi
-
(creator_code:org_t)
- 1997
- 1997
- Engelska.
-
Ingår i: ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. - 0907-4449. ; 53, s. 179-185
- Relaterad länk:
-
https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- A conceptually simple real-space convolution method has been developed which can be used to detect or enhance structural features in experimental macromolecular electron-density maps. The method has been implemented in a computer program (ESSENS). One application of the method is in selectively visualizing secondary-structure elements in multiple isomorphous replacement (MIR) maps of proteins, prior to map interpretation. This application is demonstrated for MIR maps of P2 myelin protein [Jones, Bergfors, Sedzik & Unge (1988). EMBO J. 7, 1597-1604; Cowan, Newcomer & Jones (1993). J. Mol. Biol. 230, 1225-1246] and glyoxalase I [Cameron, Olin, Ridderstrom, Mannervik & Jones (1997). In preparation]. Another application is in finding the optimal orientation and position of a known structural fragment (e.g. a protein domain or a ligand) in any type of electron-density map (real-space or phased molecular replacement). This application is demonstrated for the complex of acetylcholinesterase and the snake toxin fasciculin II [Harel, Kleywegt, Ravelli, Silman & Sussman (1995). Structure, 3, 1355-1366] where the toxin was located in a map phased using the molecular-replacement solution for the acetylcholinesterase alone.
Nyckelord
- P2 MYELIN PROTEIN; REFINEMENT
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas