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Deletion of exon I ...
Deletion of exon I of SMAD7 in mice results in altered B cell responses
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- Li, Ronggui (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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Rosendahl, Alexander (författare)
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- Brodin, Greger (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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Cheng, Alec M. (författare)
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- Åhgren, Aive (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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- Sundquist, Christina (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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Kulkarni, Sarang (författare)
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Pawson, Tony (författare)
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- Heldin, Carl-Henrik (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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- Heuchel, Rainer L. (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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(creator_code:org_t)
- 2006
- 2006
- Engelska.
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Ingår i: Journal of Immunology. - 0022-1767 .- 1550-6606. ; 176:11, s. 6777-6784
- Relaterad länk:
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http://www.jimmunol....
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The members of the TGF-beta superfamily, i.e., TGF-beta isoforms, activins, and bone morphogenetic proteins, regulate growth, differentiation, and apoptosis, both during embryonic development and during postnatal life. Smad7 is induced by the TGF-beta superfamily members and negatively modulates their signaling, thus acting in a negative, autocrine feedback manner. In addition, Smad7 is induced by other stimuli. Thus, it can fine-tune and integrate TGF-beta signaling with other signaling pathways. To investigate the functional role(s) of Smad7 in vivo, we generated mice deficient in exon I of Smad7, leading to a partial loss of Smad7 function. Mutant animals are viable, but significantly smaller on the outbred CD-1 mouse strain background. Mutant B cells showed an overactive TGF-beta signaling measured as increase of phosphorylated Smad2-positive B cells compared with B cells from wild-type mice. In agreement with this expected increase in TGF-beta signaling, several changes in B cell responses were observed. Mutant B cells exhibited increased Ig class switch recombination to IgA, significantly enhanced spontaneous apoptosis in B cells, and a markedly reduced proliferative response to LPS stimulation. Interestingly, LPS treatment reverted the apoptotic phenotype in the mutant cells. Taken together, the observed phenotype highlights a prominent role for Smad7 in development and in regulating the immune system's response to TGF-beta.
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- MEDICINE
- MEDICIN
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Li, Ronggui
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Rosendahl, Alexa ...
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Brodin, Greger
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Cheng, Alec M.
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Åhgren, Aive
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Sundquist, Chris ...
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visa fler...
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Kulkarni, Sarang
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Pawson, Tony
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Heldin, Carl-Hen ...
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Heuchel, Rainer ...
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visa färre...
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Journal of Immun ...
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Uppsala universitet