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Evaluation of two s...
Evaluation of two sample preparation methods for prostate proteome analysis
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Lexander, Helena (författare)
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- Hellman, Ulf (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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- Palmberg, Carina (författare)
- Karolinska Institutet
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- Auer, Gert (författare)
- Karolinska Institutet
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- Hellström, Magnus (författare)
- Karolinska Institutet
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- Franzén, Bo (författare)
- Karolinska Institutet
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- Jörnvall, Hans (författare)
- Karolinska Institutet
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- Egevad, Lars (författare)
- Karolinska Institutet
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(creator_code:org_t)
- Wiley, 2006
- 2006
- Engelska.
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Ingår i: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861. ; 6:13, s. 3918-3925
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
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- For laboratory techniques that require well-preserved proteins, such as 2-DE, fresh tissue must be harvested and processed as fast as possible to avoid proteolytic degradation. We describe a modified method for harvesting tissue from radical prostatectomy specimens for proteome analysis and compare it with the standard technique. Cells were scraped from cut surfaces of 11 prostate specimens. A fraction of the material was smeared on a glass slide and Giemsa stained for morphological control. The sample was collected in a medium with protease inhibitors, and the protein material was prepared for 2-DE. Filtering and Percoll centrifugation were omitted. Sample locations were noted on a specimen map. From the same area, a tissue block was harvested for comparison. The block was processed with the conventional technique including mechanical disintegration, filtering and Percoll centrifugation. Quality measures of 2-DE were similar with both methods. With the scrape sampling technique, control smears showed abundant epithelial cells and a cleaner background and processing was faster than with tissue block sampling. For proteomic analysis, the scrape sample technique has several advantages over the tissue block method.
Nyckelord
- MEDICINE
- MEDICIN
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- art (ämneskategori)
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