Allele-specific in situ hybridization (ASISH) analysis: A novel technique which resolves differential allelic usage of H19 within the same cell lineage during human placental development

• Precursory studies of H19 transcription during human foetal development have demonstrated maternally derived monoallelic expression, Analyses in extra-embryonic tissues, however, have been more equivocal, with discernible levels of expression of the pater

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genomic imprinting; H19; IGF2; trophoblast lineage; ASISH; human placenta; GROWTH FACTOR-II; IMPRINTED GENES; RELAXATION; IGF2

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