Sökning: id:"swepub:oai:DiVA.org:uu-89579" >
Collagen type I exp...
Collagen type I expression in experimental anaplastic thyroid carcinoma : regulation and relevance for tumorigenicity
-
- Dahlman, Thèrése (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Institutionen för medicinska vetenskaper
-
- Lammerts, Ellen (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Bergström, Danel (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
-
visa fler...
-
- Franzén, Åsa (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
-
- Westermark, Kerstin (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Heldin, Nils-Erik (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
-
- Rubin, Kristofer (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
visa färre...
-
(creator_code:org_t)
- 2001-12-29
- 2002
- Engelska.
-
Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 98:2, s. 186-192
- Relaterad länk:
-
https://onlinelibrar...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Fibrosis in solid malignancies plays a significant role in tumor pathophysiology. Potential mechanisms for collagen type I deposition in anaplastic thyroid carcinoma (ATC) were investigated using 6 characterized ATC cell lines. Three of these cell lines, which produced collagen type I, had, as a group, a poor tumorigenicity when inoculated in athymic mice. This group of cells generated tumors in 4 of 24 injected animals (17%). Pro-alpha 1(I) collagen mRNA-expressing carcinoma and stromal cells were interdispersed in the tumors generated by these ATC cells. By contrast, the 3 noncollagen-producing ATC cell lines were all tumorigenic with a tumor take of 60% in the whole group. In the latter tumors, pro-alpha 1(I) collagen mRNA-expressing cells were confined to the stromal compartment, well delineated from carcinoma cell islets. To study the influence of ATC cells on collagen type I synthesis by fibroblasts, we used AG 1518 diploid human fibroblasts cultured on poly-(2-hydroxyethyl methacrylate) (poly[HEMA])-coated plates. This culture condition allows the study of the effect of collagen mRNA translation in the regulation of collagen type I synthesis. Conditioned media from the 6 ATC cell lines did not influence collagen synthesis. The ATC cell line KAT-4 stimulated fibroblast synthesis of collagen type I when the two cell types were cocultured on poly[HEMA]-coated substrates. Specific inhibitors of PDGF and TGF-beta reduced the KAT 4 carcinoma cell-induced stimulation of collagen type I synthesis. Our data suggest that collagen type I production by carcinoma cells correlates negatively with tumorigenicity and that the formation of a well-defined stroma is of importance for tumor growth. Furthermore, our data suggest that tumor cells are able to stimulate collagen mRNA translation in stromal fibroblasts in direct cell-cell contact by, at least in part, transferring PDGF or TGF-beta.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas