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A novel B-cell line...
A novel B-cell line (U-2932) established from a patient with a diffuse large B-cell lymphoma following Hodgkin lymphoma
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- Amini, Rose-Marie (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Berglund, Mattias (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Rosenquist, Richard (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- von Heideman, Anne (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Lagercrantz, Svetlana (författare)
- Karolinska Institutet
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- Thunberg, Ulf (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Bergh, Jonas (författare)
- Karolinska Institutet
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- Sundström, Christer (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Glimelius, Bengt (författare)
- Karolinska Institutet,Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Enblad, Gunilla (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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(creator_code:org_t)
- 2009-07
- 2002
- Engelska.
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Ingår i: Leukemia and Lymphoma. - : Informa UK Limited. - 1042-8194 .- 1029-2403. ; 43:11, s. 2179-2189
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Little is known about mechanisms leading to secondary non-Hodgkin lymphomas (NHL) in patients treated for Hodgkin lymphoma (HL). Our aim was to characterise in detail a cell line derived from a diffuse large B-cell lymphoma (DLBCL) that had developed in a patient with relapsing HL. The cell line U-2932 was established from ascites in a patient suffering from DLBCL previously treated for HL with multiple chemotherapy regimens. Characterisation was based on morphology, immunophenotype, Epstein-Barr virus (EBV)-status, IgH gene rearrangement status, tumourigenicity, p53 sequencing, and immunohistochemical expression of p53, BCL-2 and BCL-6. The karyotype was investigated using G-banding, comparative genomic hybridisation (CGH) and spectral karyotype (SKY) analysis. This cell line shows typical morphological features of a DLBCL and grows as colonies in nude mice. It expresses a B-cell phenotype with a somatically hypermutated V(H)4-39 gene and is negative for EBV. The origin of U-2932 was confirmed by demonstrating an identical V(H)4 rearrangement in ascites from the patient. A point mutation of the tumour-suppressor gene p53 was detected in amino acid position 176 and immunohistochemical over-expression of the p53 protein was also demonstrated. U-2932 carries a complex karyotype including high-level amplifications of the chromosomal bands 18q21 and 3q27 and expresses aberrant BCL-2 and BCL-6 immunohistochemically. We were unable to investigate the clonal relationship between the original HL and U-2932. In conclusion, U-2932 is a unique B cell line established from a patient suffering from HL followed by NHL. Overexpression of BCL-2, BCL-6 and p53 may play a role in the tumourigenesis and drug resistance. This cell line may become a useful tool to better understand the mechanisms responsible for development of secondary NHL in patients treated for HL.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
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Amini, Rose-Mari ...
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Berglund, Mattia ...
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Rosenquist, Rich ...
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von Heideman, An ...
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Lagercrantz, Sve ...
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Thunberg, Ulf
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visa fler...
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Bergh, Jonas
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Sundström, Chris ...
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Glimelius, Bengt
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Enblad, Gunilla
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- Artiklar i publikationen
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Leukemia and Lym ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet