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Heterogeneous somat...
Heterogeneous somatic hypermutation status confounds the cell of origin in hairy cell leukemia
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- Thorsélius, Mia (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Walsh, Sarah H. (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Thunberg, Ulf (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Hagberg, Hans (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Sundström, Christer (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Rosenquist, Richard (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- Engelska.
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Ingår i: Leukemia research. - : Elsevier BV. - 0145-2126 .- 1873-5835. ; 29:2, s. 153-158
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Hairy cell leukemia (HCL) is thought to arise from a post-germinal center (GC) B-cell, however the exact normal counterpart remains unclear. We performed VH gene analysis of 32 HCL cases, revealing somatically mutated VH genes (<98% homology) in 27 cases and unmutated VH genes in five cases, four of which displayed germline VH genes. Intraclonal heterogeneity was evident in the majority of eight mutated HCLs investigated, although at a lower level compared to GC-derived lymphomas. A novel finding of preferential VH3-30 gene usage was detected (19% of HCLs). Our data confounds the postulated post-GC origin in HCL considering (1) the finding of unmutated HCLs, generally correlating with a pre-GC origin, and (2) the presence of intraclonal variation in mutated HCLs. The latter suggests that the transformed B-cell was frozen when it still had an active mutation process, implying a closer relation to the GC than previously assumed. Furthermore, restricted VH3-30 usage indicates that antigen selection could be a promoting factor in HCL development.
Nyckelord
- Adult
- Aged
- Aged; 80 and over
- Base Sequence
- Female
- Gene Rearrangement; B-Lymphocyte; Heavy Chain
- Humans
- Immunoglobulin Heavy Chains/analysis/*genetics
- Leukemia; B-Cell/genetics/immunology/pathology
- Leukemia; Hairy Cell/*genetics/immunology/pathology
- Male
- Middle Aged
- Molecular Sequence Data
- Research Support; Non-U.S. Gov't
- Somatic Hypermutation; Immunoglobulin/*genetics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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