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Structure and funct...
Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis
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- Johansson, Patrik (författare)
- Uppsala universitet,Strukturell molekylärbiologi
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- Castell, Alina (författare)
- Uppsala universitet,Strukturell molekylärbiologi
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- Jones, T. Alwyn (författare)
- Uppsala universitet,Strukturell molekylärbiologi
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visa fler...
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- Bäckbro, Kristina (författare)
- Uppsala universitet,Strukturell molekylärbiologi
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(creator_code:org_t)
- Wiley, 2006
- 2006
- Engelska.
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Ingår i: Protein Science. - : Wiley. - 0961-8368 .- 1469-896X. ; 15:10, s. 2300-2309
- Relaterad länk:
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https://europepmc.or...
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visa fler...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A large fraction of the Mycobacterium tuberculosis genome codes for proteins of unknown function. We here report the structure of one of these proteins, Rv0130, solved to a resolution of 1.8 angstrom. The Rv0130 monomer features a single hotdog fold composed of a highly curved beta-sheet on top of a long and a short alpha-helix. Two monomers in turn pack to form a double-hotdog-folded homodimer, similar to a large group of enzymes that use thiol esters as substrates. Rv0130 was found to contain a highly conserved R-specific hydratase motif buried deeply between the two monomers. Our biochemical studies show that the protein is able to hydrate a short trans-2-enoyl-coenzyme A moiety with a k(cat) of 1.1 x 10(2) sec(-1). The importance of the side chains of D40 and H45 for hydratase activity is demonstrated by site-directed mutagenesis. In contrast to many hotdog-folded proteins, a proline residue distorts the central helix of Rv0130. This distortion allows the creation of a long, curved tunnel, similar to the substrate-binding channels of long-chain eukaryotic hydratase 2 enzymes.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Rv0130
- Mycobacterium tuberculosis
- hydratase
- hotdog fold
- crystal structure
- Biology
- Biologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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