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High throughput ana...
High throughput analysis of tryptophan metabolites in a complex matrix using capillary electrophoresis coupled to time-of-flight mass spectrometry
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- Arvidsson, Björn (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Johannesson, Nina (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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Citterio, Attilio (författare)
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Righetti, Pier Giorgio (författare)
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- Bergquist, Jonas (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
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Ingår i: Journal of Chromatography A. - : Elsevier BV. - 0021-9673 .- 1873-3778. ; 1159:1-2, s. 154-158
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A capillary electrophoresis method for separation and detection with time-of-flight mass spectrometry is described for tryptophan metabolites in the kynurenic pathway. Tryptophan metabolites are usually difficult to detect with electrospray mass spectrometry since they have low surface activity and occur in low nanomolar to micromolar range in body fluids. Modification of the silica-wall with 1-(4-iodobutyl)4-aza-1-azoniabicyclo[2,2,2]octane iodide, also named M7C4I, has successfully been used to deactivate the fused silica wall and generate a stable reversed electroosmotic flow. Utilizing this advantage together with electrospray ionization time-of-flight mass spectrometry, which generates high resolution and fast acquisition monitoring of species, proved to be successful even for such a complex matrix like human cerebrospinal fluid.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Tryptophan
- Kynurenine
- Kynurenic acid
- Quinolinic acid
- Picolinic acid
- Capillary electrophoresis
- Mass spectrometry
- Time-of-flight
- Complex matrix
- Cerebrospinal fluid
- Chemistry
- Kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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