Sensitivity to extrinsically supplied interferon and the endogenous expression of interferon in melanoma cell lines.

• Interferons (IFNs) have been shown to Induce loss of growth potential in melanoma cell lines. However, human melanomas have shown limited responsiveness to clinical therapy with IFN. In a previous study on melanoma cell lines we found that greatest sensitivity to IFN was found in cell lines with the greatest number of copies of chromosome 9p, where the IFN gene family is located. In the present study the expression In melanoma cell lines of IFN genes, IFN receptor genes and standard control genes (beta-actin, glyceraldehyde-3-phosphate dehydrogenase, 18S rRNA and cyclophilin) was investigated using the reverse transcription-polymerase chain reaction, together with an exogenous standard (cyclophllin armoured RNA). We found that the sensitivity to extrinsically supplied IFN seems to correlate with the expression of endogenous IFN genes. The two melanoma cell lines producing the highest relative amount of IFN mRNA transcripts also demonstrated the most marked response to extrinsically supplied IFN. We hypothesize that tumours with enhanced endogenous IFN production may respond more positively to IFN treatment.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Nyckelord

Cell Division

drug effects

Chromosomes

Human

Pair 21

genetics

Chromosomes

Human

Pair 9

genetics

Gene Dosage

Humans

Interferon-alpha

biosynthesis

genetics

pharmacology

Interferon-beta

biosynthesis

genetics

pharmacology

Melanoma

genetics

metabolism

pathology

RNA

Messenger

biosynthesis

Receptor

Interferon alpha-beta

Receptors

Interferon

biosynthesis

genetics

Reverse Transcriptase Polymerase Chain Reaction

Tumor Cells

Cultured

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)