Proteomic studies in zebrafish liver cells exposed to the brominated flame retardants HBCD and TBBPA.

• Proteomic effect screening in zebrafish liver cells was performed to generate hypotheses regarding single and mixed exposure to the BFRs HBCD and TBBPA. Responses at sublethal exposure were analysed by two-dimensional gel electrophoresis followed by MALDI-TOF and FT-ICR protein identification. Mixing of HBCD and TBBPA at sublethal doses of individual substances seemed to increase toxicity. Proteomic analyses revealed distinct exposure-specific and overlapping responses suggesting novel mechanisms with regard to HBCD and TBBPA exposure. While distinct HBCD responses were related to decreased protein metabolism, TBBPA revealed effects related to protein folding and NADPH production. Overlapping responses suggest increased gluconeogenesis (GAPDH and aldolase) while distinct mixture effects suggest a pronounced NADPH production and changes in proteins related to cell cycle control (prohibitin and crk-like oncogene). We conclude that mixtures containing HBCD and TBBPA may result in unexpected effects highlighting proteomics as a sensitive tool for detecting and hypothesis generation of mixture effects.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Animals

Biological Markers

analysis

metabolism

Cell Culture Techniques

Cell Survival

drug effects

Cells

Cultured

Electrophoresis

Gel

Two-Dimensional

Environmental Monitoring

Flame Retardants

toxicity

Fourier Analysis

Hydrocarbons

Brominated

toxicity

Liver

cytology

drug effects

metabolism

Polybrominated Biphenyls

toxicity

Proteome

metabolism

Spectrometry

Mass

Matrix-Assisted Laser Desorption-Ionization

Water Pollutants

Chemical

toxicity

Zebrafish

growth & development

metabolism

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