Analysis of monosaccharide composition of mucin oligosaccharide alditols by high-performance anion-exchange chromatography.

• A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re-N-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detector. The method was applied on a mixture of neutral oligosaccharides released from mucin glycopeptides of rat small intestine by alkaline borohydride. Sugars were detected down to the nanomole range and the results were compared with monosaccharide compositional analysis performed by gas chromatography of acetylated alditols.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Other Basic Medicine (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Nyckelord

Acetylation

Animals

Anions

Boranes

Chromatography

High Pressure Liquid

Chromatography

Ion Exchange

Hydrolysis

Intestine

Small

chemistry

Monosaccharides

analysis

Mucins

chemistry

Oligosaccharides

analysis

Rats

Sugar Alcohols

analysis

Trifluoroacetic Acid

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)