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Structural features...
Structural features of cytochrome c' folding intermediates revealed by fluorescence energy-transfer kinetics.
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Lee, Jennifer C (författare)
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- Engman, Cecilia, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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Tezcan, F Akif (författare)
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Gray, Harry B (författare)
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Winkler, Jay R (författare)
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(creator_code:org_t)
- 2002-10-29
- 2002
- Engelska.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 99:23, s. 14778-82
- Relaterad länk:
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http://www.pnas.org/...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We employed fluorescence energy-transfer probes to investigate the polypeptide dynamics accompanying cytochrome c' folding. Analysis of fluorescence energy-transfer kinetics from wild-type Trp-72 or Trp-32 in a crystallographically characterized (1.78 A) Q1A/F32W/W72F mutant shows that there is structural heterogeneity in denatured cytochrome c'. Even at guanidine hydrochloride concentrations well beyond the unfolding transition, a substantial fraction of the polypeptides ( approximately 50%) adopts compact conformations (tryptophan-to-heme distance, approximately 25 A) in both pseudo-wild-type (Q1A) and mutant proteins. A burst phase (< or =5 ms) is revealed when stopped flow-triggered refolding is probed by tryptophan intensity: measurements on the Q1A protein show that approximately 75% of the Trp-72 fluorescence (83% for Trp-32) is quenched within the mixing deadtime, suggesting that most of the polypeptides have collapsed.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Amino Acids
- analysis
- Bacterial Proteins
- chemistry
- metabolism
- Crystallography
- X-Ray
- methods
- Cytochromes c1
- chemistry
- metabolism
- Energy Transfer
- Guanidine
- Kinetics
- Protein Conformation
- Protein Folding
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- chemistry
- metabolism
- Spectrometry
- Fluorescence
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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