Healing of PTFE grafts in a pig model recruit neointimal cells from different sources and do not endothelialize.

• OBJECTIVES: The aim of this study was to analyze the cellular sources for the neointima and the cell type that is lining the lumen in artificial grafts implanted in pigs. MATERIALS AND METHODS: We used polytetrafluoroethylene grafts as bypasses from the common to the external iliac arteries. The animals were sacrificed after 1, 4, 7, 14, 21, 30, 60 and 90 days. Morphological, immunohistochemical and electron microscope assessments were made. RESULTS: After 7 days a circumferential neoadventitia was formed. At day 14 isolated cellular islets of proliferating cells were observed on the luminal side of the graft without connection to the neoadventitia or the adjacent arteries. In the anastomotic regions at day 14 we observed an isolated neointima in contact with the adjacent artery. The cells lining the lumen had characteristics of both smooth muscle cells and endothelial cells. CONCLUSIONS: Our study suggests that in artificial porcine grafts, the perivascular tissue, the blood and the adjacent artery contribute to the formation of the neointima. The luminal surface is covered by a hybrid cell with both smooth muscle cell and endothelial cell properties.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

Nyckelord

Actins

metabolism

Anastomosis

Surgical

Animals

Arterial Occlusive Diseases

metabolism

pathology

surgery

Blood Vessel Prosthesis

Cell Division

Disease Models

Animal

Foreign-Body Reaction

metabolism

pathology

Iliac Artery

metabolism

surgery

ultrastructure

Immunohistochemistry

Leukocyte Count

Leukocytes

ultrastructure

Male

Microscopy

Electron

Scanning

Microscopy

Electron

Transmission

Polytetrafluoroethylene

Proliferating Cell Nuclear Antigen

metabolism

Stents

Swine

Tunica Intima

metabolism

ultrastructure

Wound Healing

physiology

von Willebrand Factor

metabolism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)