Choukroun's platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way.

• BACKGROUND: Choukroun's platelet-rich fibrin (PRF) is an autologous leukocyte- and platelet-rich fibrin biomaterial. The purpose of this study was to analyse the in vitro effects of PRF on human bone mesenchymal stem cells (BMSC), harvested in the oral cavity after preimplant endosteal stimulation. MATERIALS AND METHODS: BMSCs from primary cultures were cultivated with or without a PRF membrane originating from the same donor as for the cells, in proliferation or osteoblastic differentiation conditions. After 7 days, the PRF membranes were removed. A series of cultures were performed using 2 PRF membranes, in order to measure the dose-dependent effect. Cell counts, cytotoxicity tests, alkaline phosphatase (ALP) activity quantification, Von Kossa staining and mineralisation nodules counts were performed at 3, 7, 14, 21 and 28 days. A last independent series was carried on up to 14 days, for a morphological scanning electron microscope (SEM) observation. RESULTS: PRF generated a significant stimulation of the BMSC proliferation and differentiation throughout the experimental period. This effect was dose-dependent during the first weeks in normal conditions, and during the whole experimentation in differentiation conditions. The cultures without PRF in differentiation conditions did not rise above the degree of differentiation of the cultures in normal conditions with 1 or 2 PRF up to the 14th and 28th day, respectively. The SEM culture analysis at day 14 allowed to show the mineralisation nodules which were more numerous and more structured in the groups with PRF compared to the control groups. DISCUSSION AND CONCLUSIONS: This double contradictory proliferation/differentiation result may be due to the numerous components of PRF, particularly the presence of leukocytes: any culture with PRF is in fact a coculture with leukocytes. It could be the source of differential geographic regulation processes within the culture. The combination of oral BMSC and PRF might offer many potential clinical and biotechnological applications, and deserves new studies.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Odontologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dentistry (hsv//eng)

Nyckelord

Alkaline Phosphatase

analysis

Biocompatible Materials

pharmacology

Blood Platelets

physiology

Calcification

Physiologic

physiology

Cell Count

Cell Differentiation

drug effects

Cell Proliferation

drug effects

Cell Survival

drug effects

Cells

Cultured

Coculture Techniques

Coloring Agents

diagnostic use

Cytotoxicity Tests

Immunologic

Dose-Response Relationship

Drug

Fibrin

pharmacology

Humans

Leukocytes

physiology

Male

Maxilla

cytology

Mesenchymal Stem Cells

drug effects

Microscopy

Electron

Scanning

Middle Aged

Osteoblasts

drug effects

Osteotomy

methods

Spectrophotometry

Tetrazolium Salts

diagnostic use

Thiazoles

diagnostic use

Time Factors

Tissue and Organ Harvesting

methods

Trypan Blue

diagnostic use

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)