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The ability of surf...
The ability of surface characteristics of materials to trigger leukocyte tissue factor expression.
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Fischer, Marion (författare)
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Sperling, Claudia (författare)
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- Tengvall, Pentti (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap,Institute of Clinical Sciences, Department of Biomaterials
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Werner, Carsten (författare)
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(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- Engelska.
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Ingår i: Biomaterials. - : Elsevier BV. - 1878-5905 .- 0142-9612. ; 31:9, s. 2498-507
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Biomaterial-induced thrombosis is usually attributed to blood coagulation initiated by contact phase and platelet-related reactions. Considering the major role of extrinsic initiation in blood coagulation in vivo, we studied the material related-induction of this pathway by investigating the relevance of surface properties for the expression of Tissue Factor (TF), a critical initiator of the extrinsic pathway of coagulation. We incubated materials with self-assembled monolayers of alkylthiols (SAMs) displaying various ratios of -CH(3), -OH, and -COOH terminations with fresh heparinized whole human blood in vitro. The transcription of TF-mRNA in leukocytes showed clear differences in relation to surface properties and increased over time. In addition, a positive correlation between TF transcription and its presence on leukocytes, granulocyte activation, and complement activation was found. Cells displaying the highest TF expression after material contact had significantly lower intracellular TF, pointing to previous TF release. Yet under the conditions of our whole blood incubation set-up within the limited time frame the observed initiation of the extrinsic pathway did not trigger blood coagulation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- Antithrombins
- metabolism
- Biocompatible Materials
- pharmacology
- Biological Assay
- Cell Adhesion
- drug effects
- Complement Activation
- drug effects
- Complement C3
- immunology
- Factor XIIa
- metabolism
- Flow Cytometry
- Humans
- Kallikreins
- blood
- Leukocytes
- cytology
- drug effects
- metabolism
- Materials Testing
- Platelet Adhesiveness
- drug effects
- Surface Properties
- drug effects
- Thrombin
- metabolism
- Thromboplastin
- genetics
- metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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