Surface plasmon resonance detection of blood coagulation and platelet adhesion under venous and arterial shear conditions.

• A surface plasmon resonance (SPR) based flow chamber device was designed for real time detection of blood coagulation and platelet adhesion in platelet rich plasma (PRP) and whole blood. The system allowed the detection of surface interactions throughout the 6mm length of the flow chamber. After deposition of thromboplastin onto a section of the sensor surface near the inlet of the flow chamber, coagulation was detected downstream of this position corresponding to a SPR signal of 7 to 8 mRIU (7 to 8 ng/mm2). A nonmodified control surface induced coagulation 3.5 times slower. Platelet adhesion to gold and fibrinogen coated surfaces in the magnitude of 1.25 and 1.66 mRIU was also shown with platelets in buffer, respectively. SPR responses obtained with PRP and whole blood on surfaces that were methylated or coated with von Willebrand factor (vWF), fibrinogen, or collagen, coincided well with platelet adhesion as observed with fluorescence microscopy in parallel experiments. The present SPR detection equipped flow chamber system is a promising tool for studies on coagulation events and blood cell adhesion under physiological flow conditions, and allows monitoring of short-range surface processes in whole blood.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Odontologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dentistry (hsv//eng)

Keyword

Biosensing Techniques

instrumentation

methods

Blood Coagulation

physiology

Blood Vessels

cytology

physiology

Cell Culture Techniques

instrumentation

methods

Cells

Cultured

Equipment Design

Equipment Failure Analysis

Flow Injection Analysis

instrumentation

methods

Humans

Platelet Adhesiveness

physiology

Reproducibility of Results

Sensitivity and Specificity

Shear Strength

Surface Plasmon Resonance

instrumentation

methods

MEDICINE

Publication and Content Type

ref (subject category)

art (subject category)