Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine.

• The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

Nyckelord

Administration

Cutaneous

Animals

Hepacivirus

genetics

Mice

Mice

Inbred BALB C

Microinjections

Needles

T-Lymphocytes

Cytotoxic

immunology

Vaccines

DNA

administration & dosage

immunology

Viral Proteins

genetics

immunology

Viral Vaccines

administration & dosage

immunology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)