Sensitive liquid chromatography-electrospray mass spectrometry allows for the analysis of the O-glycosylation of immunoprecipitated proteins from cells or tissues: application to MUC1 glycosylation in cancer.

• We have analyzed the structures of the glycans on immunoprecipitated proteins from small amounts of cell or tissue lysates, by liquid-chromatography electrospray mass spectrometry (LC-ESI-MS) and MS/MS. The sensitive and specific method was applied to the analysis of the O-glycosylation of MUC1 in breast, prostate and gastric cancer, including analysis of a patient tumor specimen. The method will be applicable for the glycosylation analysis of individual proteins.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Nyckelord

Carbohydrate Conformation

Carbohydrate Sequence

Cell Line

Chromatography

Liquid

methods

Glycosylation

Humans

Immunoprecipitation

Molecular Sequence Data

Mucin-1

chemistry

metabolism

Neoplasms

chemistry

diagnosis

metabolism

Sensitivity and Specificity

Spectrometry

Mass

Electrospray Ionization

methods

Publikations- och innehållstyp

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