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Murine Gammaretrovi...
Murine Gammaretrovirus Group G3 Was Not Found in Swedish Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome and Fibromyalgia
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- Elfaitouri, A (författare)
- Uppsala universitet,Klinisk virologi
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- Shao, XW (författare)
- Uppsala universitet,Klinisk virologi
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- Ulfstedt, Johan Mattsson (författare)
- Uppsala universitet,Klinisk virologi
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- Muradrasoli, Shaman (författare)
- Uppsala universitet,Klinisk virologi
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- Wiener, AB (författare)
- Uppsala universitet,Klinisk virologi
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- Golbob, S (författare)
- Uppsala universitet,Klinisk virologi
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- Öhrmalm, Christina (författare)
- Uppsala universitet,Klinisk virologi
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- Matousek, Markus, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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- Zachrisson, Olof, 1963 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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- Gottfries, Carl-Gerhard, 1928 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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- Blomberg, Jonas (författare)
- Uppsala universitet,Klinisk virologi
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Matousek, Michael (författare)
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(creator_code:org_t)
- Public Library of Science (PLoS), 2011
- 2011
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:10
- Relaterad länk:
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https://journals.plo...
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https://gup.ub.gu.se...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: The recent report of gammaretroviruses of probable murine origin in humans, called xenotropic murine retrovirus related virus (XMRV) and human murine leukemia virus related virus (HMRV), necessitated a bioinformatic search for this virus in genomes of the mouse and other vertebrates, and by PCR in humans. Results: Three major groups of murine endogenous gammaretroviruses were identified. The third group encompassed both exogenous and endogenous Murine Leukemia Viruses (MLVs), and most XMRV/HMRV sequences reported from patients suffering from myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Two sensitive real-time PCRs for this group were developed. The predicted and observed amplification range for these and three published XMRV/HMRV PCRs demonstrated conspicuous differences between some of them, partly explainable by a recombinatorial origin of XMRV. Three reverse transcription real-time PCRs (RTQPCRs), directed against conserved and not overlapping stretches of env, gag and integrase (INT) sequences of XMRV/HMRV were used on human samples. White blood cells from 78 patients suffering from ME/CFS, of which 30 patients also fulfilled the diagnostic criteria for fibromyalgia (ME/CFS/FM) and in 7 patients with fibromyalgia (FM) only, all from the Gothenburg area of Sweden. As controls we analyzed 168 sera from Uppsala blood donors. We controlled for presence and amplifiability of nucleic acid and for mouse DNA contamination. To score as positive, a sample had to react with several of the XMRV/HMRV PCRs. None of the samples gave PCR reactions which fulfilled the positivity criteria. Conclusions: XMRV/HMRV like proviruses occur in the third murine gammaretrovirus group, characterized here. PCRs developed by us, and others, approximately cover this group, except for the INT RTQPCR, which is rather strictly XMRV specific. Using such PCRs, XMRV/HMRV could not be detected in PBMC and plasma samples from Swedish patients suffering from ME/CFS/FM, and in sera from Swedish blood donors.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Elfaitouri, A
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Shao, XW
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Ulfstedt, Johan ...
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Muradrasoli, Sha ...
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Wiener, AB
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Golbob, S
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Öhrmalm, Christi ...
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Matousek, Markus ...
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Zachrisson, Olof ...
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Gottfries, Carl- ...
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Blomberg, Jonas
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Matousek, Michae ...
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- MEDICIN OCH HÄLSOVETENSKAP
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och Neurovetenskaper
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PLOS ONE
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Göteborgs universitet
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Uppsala universitet