Proteomic study of the mucin granulae in an intestinal goblet cell model.

• Goblet cells specialize in producing and secreting mucus with its main component, mucins. An inducible goblet-like cell line was used for the purification of the mucus vesicles stored in these cells by density gradient ultracentrifugation, and their proteome was analyzed by nanoLC-MS and MS/MS. Although the density of these vesicles coincides with others, it was possible to reveal a number of proteins that after immunolocalization on colon tissue and functional analyses were likely to be linked to the MUC2 vesicles. Most of the proteins were associated with the vesicle membrane or their outer surface. The ATP6AP2, previously suggested to be associated with vesicular proton pumps, was colocalized with MUC2 without other V-ATPase proteins and, thus, probably has roles in mucin vesicle function yet to be discovered. FAM62B, known to be a calcium-sensitive protein involved in vesicle fusion, also colocalized with the MUC2 vesicles and is probably involved in unknown ways in the later events of the MUC2 vesicles and their secretion.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Cells

Cultured

Centrifugation

Density Gradient

Colon

Sigmoid

cytology

metabolism

secretion

Goblet Cells

metabolism

secretion

Humans

Mucin-2

chemistry

isolation & purification

metabolism

secretion

Peptide Fragments

chemistry

Principal Component Analysis

Protein Binding

Protein Interaction Mapping

Protein Transport

Proteomics

R-SNARE Proteins

metabolism

Receptors

Cell Surface

chemistry

metabolism

Secretory Vesicles

metabolism

Synaptotagmins

chemistry

metabolism

Vacuolar Proton-Translocating ATPases

chemistry

metabolism

rab3A GTP-Binding Protein

metabolism

Publikations- och innehållstyp

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