Binding of the galactose-specific Pseudomonas aeruginosa lectin, PA-I, to glycosphingolipids and other glycoconjugates.

• The carbohydrate-binding specificity of Pseudomonas aeruginosa lectin I (PA-I) in iodinated or biotinylated form was studied. A large number of glycosphingolipids, as well as some glycoproteins and neoglycoproteins were used as ligands. Also, inhibition by free saccharides of PA-I binding to glycosphingolipids was tested. It was found that the lectin binds most strongly to terminal and nonsubstituted Gal alpha 3Gal- or Gal alpha 4Gal-structures.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Other Basic Medicine (hsv//eng)

Nyckelord

Adhesins

Bacterial

Bacterial Adhesion

Bacterial Proteins

metabolism

Carbohydrate Sequence

Chromatography

Thin Layer

Galactose

metabolism

Glycoconjugates

metabolism

Glycoproteins

metabolism

Glycosphingolipids

metabolism

Lectins

metabolism

Molecular Sequence Data

Protein Binding

Pseudomonas

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)