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Interaction between...
Interaction between the anticancer drug cisplatin and the copper chaperone Atox1 in human melanoma cells
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- Palm-Espling, Maria (författare)
- Umeå universitet,Kemiska institutionen,Pernilla Wittung-Stafshede
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- Lundin, Christina (författare)
- Umeå universitet,Kirurgi,Peter Naredi
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- Björn, Erik (författare)
- Umeå universitet,Kemiska institutionen
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- Naredi, Peter, 1955 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery,Kirurgi, Sahlgrenska sjukhuset, Göteborgs universitet
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- Wittung-Stafshede, Pernilla (författare)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- Bentham Science Publishers Ltd. 2014
- 2014
- Engelska.
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Ingår i: Protein Peptide Letters. - : Bentham Science Publishers Ltd.. - 0929-8665 .- 1875-5305. ; 21:1, s. 63-68
- Relaterad länk:
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- Cisplatin (CisPt) is one of the most common anticancer drugs used against many severe forms of cancers. However, treatment with this drug causes many side effects and often, it results in the development of cell resistance. A majority of side effects as well as cell resistance are thought to develop due to CisPt interactions with proteins prior to reaching the nucleus and the DNA target. The copper (Cu) transport proteins Ctr1 and ATP7A/B have been implicated in cellular resistance of CisPt, possibly exporting the drug out of the cell. Recent in vitro work demonstrated that CisPt also interacts with the cytoplasmic Cu-chaperone Atox1, binding in or near the Cu-binding site, without expulsion of bound Cu. Whereas Ctr1 and ATP7B interactions with CisPt have been shown in vivo or ex vivo, there is no such information for Atox1-CisPt interactions. To address this, we developed a method to probe if CisPt interacts with Atox1 in human melanoma cells. Atox1-specific antibodies were linked to magnetic beads and used to immune-precipitate Atox1 from melanoma cells that had been pre-exposed to CisPt. Analysis of extracted Atox1 with inductively coupled plasma mass spectrometry demonstrated the presence of Pt in the protein fraction. Thus, CisPt-exposed human melanoma cells contain Atox1 molecules that bind some derivative of CisPt. This study gives the first indication for the intracellular presence of Atox1-CisPt complexes ex vivo. © 2014 Bentham Science Publishers.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Anticancer
- Atox1
- Cisplatin
- Copper chaperone
- Immunoprecipitation
- Resistance
- antineoplastic agent
- Atox1 protein
- cell protein
- chaperone
- copper
- unclassified drug
- article
- binding site
- cell lysate
- cytoplasm
- ex vivo study
- gene targeting
- human
- human cell
- in vitro study
- in vivo study
- mass spectrometry
- melanoma
- protein interaction
- protein transport
- Copper-chaperone
- biologisk kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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