Evidence for an expression of blood group A antigen on platelet glycoproteins IV and V.

• Blood group ABO antigens are known to be carried by several platelet glycoproteins (GP), e.g. GPIb, GPIIa, GPIIb, GPIIa and PECAM. Beside these proteins, we recently observed that blood group A antigen was also expressed on some other uncharacterized platelet proteins (70-90 kDa) having electrophoretic mobilities closely resembling those of GPIV and GPV. These findings prompted us further to characterize these latter ABO-expressing platelet proteins. By antigen capture ELISA, wherein the monoclonal antibodies (mAbs) CLB-IVC7 and CLB-SWI6 were used to hold the corresponding antigens GPIV and GPV, human anti-A specifically bound to these proteins derived from A1-platelets; neither GPIV nor GPV derived from A2-, B- or O-platelets bound anti-A. In a Western blot assay using immunoprecipitated GPIV and GPV as antigens, mAb anti-A immunostained GPIV and GPV precipitated from A1, but not from A2 and O platelets. These results conclusively demonstrate that blood group A antigen is expressed on platelet GPIV and GPV.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

ABO Blood-Group System

analysis

Adult

Animals

Antibodies

Monoclonal

immunology

Antigens

CD36

chemistry

immunology

Antigens

Human Platelet

chemistry

immunology

Blood Platelets

chemistry

Enzyme-Linked Immunosorbent Assay

Female

Glycosylation

Humans

Infant

Newborn

Mice

Platelet Glycoprotein GPIb-IX Complex

chemistry

immunology

Protein Processing

Post-Translational

Thrombocytopenia

congenital

immunology

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