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Subcellular localiz...
Subcellular localization of an ATPase in anammox bacteria using proteomics and immunogold electron microscopy
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Karlsson, R. (författare)
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Karlsson, A. (författare)
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- Bäckman, Ola, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Johansson, Bengt R, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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- Hulth, Stefan, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2014-04-07
- 2014
- Engelska.
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Ingår i: Fems Microbiology Letters. - : Oxford University Press (OUP). - 0378-1097. ; 354:1, s. 10-18
- Relaterad länk:
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https://academic.oup...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Anaerobic ammonium oxidation (anammox) has received significant attention during optimization of waste-water treatment and constitutes an important pathway for the removal of bioavailable nitrogen from natural environments. Studies of key catabolic enzymes indicate that the anammox reaction takes place inside the anammoxosome, an organelle-like membranous compartment of anammox bacteria. The anammoxosome has also been suggested as a site for ATP synthesis. A lipid-based protein immobilization technique, previously used to identify proteins essential for the anammox reaction, was in this study used to select linear epitopes for antibodies specifically targeted against an identified ATPase. The approach of using proteomics and bioinformatics as tools for selecting antibody targets for immunolocalization provides an important alternative to traditional methods for selection of specific antibodies. Immunogold electron microscopy and statistical evaluations indicated that the antibodies against the ATPase were exclusively found associated with the anammoxosome membrane. This provides strong evidence for ATP synthesis by an intracellular proton motive force in anammox bacteria. Within prokaryotes, an ATP synthase associated with an intracellular compartment is a feature unique for anammox bacteria.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- ATPase
- anammoxosome
- linear epitope
- immunogold EM
- anammox bacteria
- ANAEROBIC AMMONIUM OXIDATION
- CANDIDATUS KUENENIA STUTTGARTIENSIS
- OXIDIZING BACTERIA
- INTRACYTOPLASMIC COMPARTMENT
- CELL
- PROTEIN
- MICROORGANISMS
- PLAN
- Microbiology
- MICROBIOLOGY
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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