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Development of inte...
Development of intestinal ion-transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar
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- Sundh, Henrik, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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Nilsen, T. O. (författare)
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- Lindström, Jenny (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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- Hasselberg Frank, Linda, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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Stefansson, S. O. (författare)
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McCormick, S. D. (författare)
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- Sundell, Kristina, 1959 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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(creator_code:org_t)
- 2014-09-26
- 2014
- Engelska.
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Ingår i: Journal of Fish Biology. - : Wiley. - 0022-1112. ; 85:4, s. 1227-1252
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- This study investigated the expression of ion transporters involved in intestinal fluid absorption and presents evidence for developmental changes in abundance and tissue distribution of these transporters during smoltification and seawater (SW) acclimation of Atlantic salmon Salmo salar. Emphasis was placed on Na+, K+-ATPase (NKA) and Na+, K+, Cl- co-transporter (NKCC) isoforms, at both transcriptional and protein levels, together with transcription of chloride channel genes. The nka alpha 1c was the dominant isoform at the transcript level in both proximal and distal intestines; also, it was the most abundant isoform expressed in the basolateral membrane of enterocytes in the proximal intestine. This isoform was also abundantly expressed in the distal intestine in the lower part of the mucosal folds. The protein expression of intestinal Nka alpha 1c increased during smoltification. Immunostaining was localized to the basal membrane of the enterocytes in freshwater (FW) fish, and re-distributed to a lateral position after SW entry. Two other Nka isoforms, alpha 1a and alpha 1b, were expressed in the intestine but were not regulated to the same extent during smoltification and subsequent SW transfer. Their localization in the intestinal wall indicates a house-keeping function in excitatory tissues. The absorptive form of the NKCC-like isoform (sub-apically located NKCC2 and/or Na+, Cl(-)co-transporter) increased during smoltification and further after SW transfer. The cellular distribution changed from a diffuse expression in the sub-apical regions during smoltification to clustering of the transporters closer to the apical membrane after entry to SW. Furthermore, transcript abundance indicates that the mechanisms necessary for exit of chloride ions across the basolateral membrane and into the lateral intercellular space are present in the form of one or more of three different chloride channels: cystic fibrosis transmembrane conductance regulator I and II and chloride channel 3.
Ämnesord
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
Nyckelord
- cystic fibrosis transmembrane conductance regulator
- fluid absorption
- Na+/K+-ATPase
- Na+/K+/2Cl(-)
- PARR-SMOLT TRANSFORMATION
- K-CL COTRANSPORTER
- CATION-CHLORIDE-COTRANSPORTERS
- TROUT ONCORHYNCHUS-MYKISS
- RAINBOW-TROUT
- FRESH-WATER
- MARINE TELEOST
- NA+/K+-ATPASE
- BROWN TROUT
- OSMOREGULATORY PHYSIOLOGY
- Fisheries
- Marine & Freshwater Biology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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