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Intestinal MUC2 muc...
Intestinal MUC2 mucin supramolecular topology by packing and release resting on D3 domain assembly.
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- Nilsson, Harriet E. (författare)
- Karolinska Institutet,KTH,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Strukturell bioteknik
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- Ambort, Daniel, 1978 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Core Facilities, Mammalian Protein Expression,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Core Facilities, Mammalian Protein Expression
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- Bäckström, Malin, 1967 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Thomsson, Elisabeth, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Mammalian Protein Expression,Core Facilities, Mammalian Protein Expression
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- Koeck, Philip J. B. (författare)
- Karolinska Institutet,KTH,Strukturell bioteknik,Department of Biosciences and Nutrition, Karolinska Institutet
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- Hansson, Gunnar C., 1951 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Hebert, Hans (författare)
- Karolinska Institutet,KTH,Strukturell bioteknik,epartment of Biosciences and Nutrition, Karolinska Institutet
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(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- Engelska.
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Ingår i: Journal of molecular biology. - : Elsevier BV. - 1089-8638 .- 0022-2836. ; 426:14, s. 2567-79
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Abstract
Ämnesord
Stäng
- MUC2 is the major gel-forming mucin of the colon forming a protective gel barrier organized into an inner stratified and an outer loose layer. The MUC2 N-terminus (D1-D2-D'D3 domains) has a dual function in building a net-like structure by disulfide-bonded trimerization and packing the MUC2 polymer into an N-terminal concatenated polygonal platform with the C-termini extending perpendicularly by pH- and calcium-dependent interactions. We studied the N-terminal D'D3 domain by producing three recombinant variants, with or without Myc tag and GFP (green fluorescent protein), and analyzed these by gel filtration, electron microscopy and single particle image processing. The three variants were all trimers when analyzed upon denaturing conditions but eluted as hexamers upon gel filtration under native conditions. Studies by electron microscopy and three-dimensional maps revealed cage-like structures with 2- and 3-fold symmetries. The structure of the MUC2 D3 domain confirms that the MUC2 mucin forms branched net-like structures. This suggests that the MUC2 mucin is stored with two N-terminal concatenated ring platforms turned by 180° against each other, implicating that every second unfolded MUC2 net in mature mucus is turned upside down.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Animals
- CHO Cells
- Colon
- chemistry
- metabolism
- Cricetulus
- Green Fluorescent Proteins
- genetics
- metabolism
- Humans
- Imaging
- Three-Dimensional
- Intestines
- metabolism
- Microscopy
- Electron
- Mucin-2
- chemistry
- genetics
- metabolism
- Protein Conformation
- Protein Structure
- Tertiary
- Recombinant Proteins
- genetics
- metabolism
- Von-Willebrand-Factor
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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