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Reduction of lectin...
Reduction of lectin valency drastically changes glycolipid dynamics in membranes but not surface avidity.
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Arnaud, Julie (author)
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Claudinon, Julie (author)
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Tröndle, Kevin (author)
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Trovaslet, Marie (author)
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- Larson, Göran, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
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Thomas, Aline (author)
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Varrot, Annabelle (author)
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Römer, Winfried (author)
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Imberty, Anne (author)
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Audfray, Aymeric (author)
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(creator_code:org_t)
- 2013-07-23
- 2013
- English.
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In: ACS chemical biology. - : American Chemical Society (ACS). - 1554-8937 .- 1554-8929. ; 8:9, s. 1918-24
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https://doi.org/10.1...
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Abstract
Subject headings
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- Multivalency is proposed to play a role in the strong avidity of lectins for glycosylated cell surfaces and also in their ability to affect membrane dynamics by clustering glycosphingolipids. Lectins with modified valency were designed from the β-propeller fold of Ralstonia solanacearum lectin (RSL) that presents six fucose binding sites. After identification of key amino acids by molecular dynamics calculations, two mutants with reduced valency were produced. Isothermal titration calorimetry confirmed the loss of three high affinity binding sites for both mutants. Crystal structures indicated that residual low affinity binding occurred in W76A but not in R17A. The trivalent R17A mutant presented unchanged avidity toward fucosylated surfaces, when compared to hexavalent RSL. However, R17A is not able anymore to induce formation of membrane invaginations on giant unilamellar vesicules, indicating the crucial role of number of binding sites for clustering of glycolipids. In the human lung epithelial cell line H1299, wt-RSL is internalized within seconds whereas the kinetics of R17A uptake is largely delayed. Neolectins with tailored valency are promising tools to study membrane dynamics.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Bacterial Proteins
- chemistry
- metabolism
- Cell Line
- Crystallography
- X-Ray
- Glycolipids
- chemistry
- metabolism
- Humans
- Lectins
- chemistry
- metabolism
- Molecular Dynamics Simulation
- Ralstonia solanacearum
- chemistry
- cytology
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Arnaud, Julie
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Claudinon, Julie
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Tröndle, Kevin
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Trovaslet, Marie
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Larson, Göran, 1 ...
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Thomas, Aline
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show more...
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Varrot, Annabell ...
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Römer, Winfried
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Imberty, Anne
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Audfray, Aymeric
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Clinical Laborat ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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ACS chemical bio ...
- By the university
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University of Gothenburg