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Interleukin-26 in A...
Interleukin-26 in Antibacterial Host Defense of Human Lungs Effects on Neutrophil Mobilization
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- Che, K. F. (författare)
- Karolinska Institutet
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- Tengvall, Sara, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine
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- Levanen, B. (författare)
- Karolinska Institutet
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Silverpil, E. (författare)
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- Smith, Margaretha E. (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine
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Awad, M. (författare)
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Vikstrom, M. (författare)
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- Palmberg, L. (författare)
- Karolinska Institutet
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- Qvarfordt, Ingemar, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine
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- Skold, M. (författare)
- Karolinska Institutet
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- Lindén, Anders, 1961 (författare)
- Karolinska Institutet,Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine
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(creator_code:org_t)
- 2014
- 2014
- Engelska.
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Ingår i: American Journal of Respiratory and Critical Care Medicine. - 1073-449X .- 1535-4970. ; 190:9, s. 1022-1031
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Rationale: The role of the presumed Th17 cytokine IL-26 in antibacterial host defense of the lungs is not known. Objectives: To characterize the role of IL-26 in antibacterial host defense of human lungs. Methods: Intrabronchial exposure of healthy volunteers to endotoxin and vehicle was performed during bronchoscopy and bronchoalveolar lavage (BAL) samples were harvested. Intracellular IL-26 was detected using immunocytochemistry and immunocytofluorescence. This IL-26 was also detected using flow cytometry, as was its receptor complex. Cytoldnes and phosphorylated signal transducer and activator of transcription (STAT) 1 plus STAT3 were quantified using ELISA. Gene expression was analyzed by real-time polymerase chain reaction and neutrophil migration was assessed in vitro. Measurements and Main Results: Extracellular IL-26 was detected in BAL samples without prior exposure in vivo and was markedly increased after endotoxin exposure. Alveolar macrophages displayed gene expression for, contained, and released IL-26. Th and cytotoxic T cells also contained IL-26. In the BAL samples, IL-26 concentrations and innate effector cells displayed a correlation. Recombinant IL-26 potentiated neutrophil chemotaxis induced by IL-8 and fMLP but decreased chemokinesis for neutrophils. Myeloperoxidase in conditioned media from neutrophils was decreased. The IL-26 receptor complex was detected in neutrophils and IL-26 decreased phosphorylated STAT3 in these cells. In BAL and bronchial epithelial cells, IL-26 increased gene expression of the IL-26 receptor complex and STAT1 plus STAT3. Finally, IL-26 increased the release of neutrophil-mobilizing cytokines in BAL but not in epithelial cells. Conclusions: This study implies that alveolar macrophages produce IL-26, which stimulates receptors on neutrophils and focuses their mobilization toward bacteria and accumulated immune cells in human lungs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- IL-10
- infection
- inflammation
- macrophage
- T cell
- COMMUNITY-ACQUIRED PNEUMONIA
- T-CELLS
- AIRWAY INFLAMMATION
- CONVERTING-ENZYME
- EPITHELIAL-CELLS
- FAMILY-MEMBERS
- IL-10 FAMILY
- TNF-ALPHA
- GM-CSF
- RECRUITMENT
- Critical Care Medicine
- Respiratory System
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Che, K. F.
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Tengvall, Sara, ...
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Levanen, B.
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Silverpil, E.
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Smith, Margareth ...
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Awad, M.
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visa fler...
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Vikstrom, M.
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Palmberg, L.
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Qvarfordt, Ingem ...
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Skold, M.
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Lindén, Anders, ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
- Artiklar i publikationen
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American Journal ...
- Av lärosätet
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Göteborgs universitet
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Karolinska Institutet