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Structural characte...
Structural characterization of non-acid glycosphingolipids in kidneys of single blood group O and A pigs.
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Holgersson, Jan (author)
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Jovall, Per-Åke (author)
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Samuelsson, Bo, 1942 (author)
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- Breimer, Michael, 1951 (author)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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(creator_code:org_t)
- 1990
- 1990
- English.
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In: Journal of biochemistry. - 0021-924X. ; 108:5, s. 766-77
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Total non-acid glycosphingolipids were isolated from the kidneys of single pigs serologically typed on their red blood cells as blood groups O and A. Glycolipid species were purified by HPLC and structurally characterized by thin-layer chromatography, mass spectrometry, proton NMR spectroscopy, degradation analysis, and reactivity with various monoclonal antibodies, Gal alpha 1-4Gal-specific E. coli bacteria, and lectins. Glucosyl-, globotriaosyl-, and globotetraosylceramides were the predominant molecular species with lactosyl- and globopentaosylceramides (IV3GalGb4Cer) as abundant constituents too. Small amounts of galactosyl- and digalactosylceramides were also present. In the blood group O pig kidneys, blood group H antigens based on four different core saccharides (types 1, 2, 4, and lactosyl core) were identified and the major blood group structure was V2FucIV3Gal-Gb4Cer. In the kidneys from the blood group A pig the corresponding blood group A antigens were found and in addition, a type 3 chain blood group A antigen was indicated by mass spectrometry and by its reactivity with a monoclonal antibody. Trace amounts of the type 2 chain-based X and Y antigens were found while blood group B antigens and the type 1 chain based Lewis antigens could not be detected. The ceramide part of the glycolipids was mainly composed of dihydroxy 18:0 long chain bases and non-hydroxy 16:0-24:0 fatty cids.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Keyword
- ABO Blood-Group System
- immunology
- Animals
- Blood Transfusion
- Carbohydrate Sequence
- Chromatography
- High Pressure Liquid
- Chromatography
- Thin Layer
- Glycosphingolipids
- chemistry
- immunology
- Kidney
- chemistry
- immunology
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Models
- Biological
- Molecular Sequence Data
- Swine
Publication and Content Type
- ref (subject category)
- art (subject category)
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