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Chemical characteri...
Chemical characterization of penta-, hexa-, hepta-, octa-, and nonaglycosylceramides of rat small intestine having a globoside-like terminus.
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Ångström, Jonas, 1950 (författare)
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- Breimer, Michael, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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Falk, K E (författare)
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visa fler...
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- Hansson, Gunnar C., 1951 (författare)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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Karlsson, K A (författare)
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Leffler, H (författare)
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visa färre...
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(creator_code:org_t)
- 1982
- 1982
- Engelska.
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Ingår i: The Journal of biological chemistry. - 0021-9258. ; 257:2, s. 682-8
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- A novel series of glycosphingolipids has been isolated from the nonepithelial part of rat small intestine. A mixed fraction containing 3 major components corresponding to glycolipids with 5, 6, and 7 sugars and 2 minor components with 8 and 9 sugars was characterized. The structure of the major components was deduced by mass spectrometry and proton NMR spectroscopy of nondegraded permethylated and permethylated-reduced (LiAlH4) derivatives and gas-liquid chromatography of degradation products of native, permethylated, and permethylated-reduced glycolipids. The structures of the penta-, hexa-, and hepta-glycosylceramides were found to be GalNAcp beta 1 leads to (3Galp alpha 1 leads to)2-44Galp beta 1 leads to 4Glcp beta 1 leads to 1Cer. By analogy reasoning, supported by mass spectrometry, the octa- and nonaglycosylceramides were concluded to have 1 and 2 additional internal leads to 3Galp alpha 1 leads to 3 structures, respectively. A pentaglycosylceramide fraction from another rat strain was also isolated. The NMR spectra were in agreement with 2 isomeric structures of which 1 had the internal alpha 1 leads to 4 linkage replaced by an alpha 1 leads to 3 linkage. The fatty acids of all components were nonhydroxy 16:0 to 24:0 acids with the 18:0 homologue as dominating species. The major base was sphingosine and possibly monohydroxy 18:1 base in the larger glycolipids. This is a novel series of structures with a terminal saccharide identical with isoglobotetraoxylceramide (cytolipin R). The glycosyltransferase for the terminal GalNAc beta 1 leads to 3 of cytolipin R may possibly be identical with the enzyme adding the terminal sugar of this novel series. This is supported by the presence in the same tissue of probable precursor glycolipids with 4 to 8 hexoses.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Animals
- Carbohydrate Conformation
- Carbohydrate Sequence
- Globosides
- analysis
- Glycosphingolipids
- analysis
- Intestine
- Small
- analysis
- Mass Spectrometry
- Models
- Molecular
- Rats
- Rats
- Inbred Strains
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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