Glycosphingolipids of rat tissues. Different composition of epithelial and nonepithelial cells of small intestine.

• The epithelial cells of rat small intestine (jejunum-ileum) were separated from their supporting stroma (residue). Total nonacid and acid glycosphingolipids were prepared from the two compartments. The acetylated nonacid glycolipids were separated into 10-12 fractions by column chromatography. These were analyzed by chromatographic methods, mass spectrometry, and proton NMR spectroscopy and compared with glycolipids isolated from whole rat small intestine. The sialic acid-containing glycosphingolipids were compared in the same way without subfractionation. At least 37 different glycosphingolipids (different carbohydrate moieties) were found, 23 in the nonepithelial residue and 17 in the epithelial cells of one rat strain. In a second rat strain, another 4 structures were detected. The glycosphingolipids of epithelial cells and nonepithelial residue were distinctly different. Glucosylceramide, lactosylceramide, and globotriaosylceramide were found in both compartments, while isoglobotriaosylceramide was restricted to the nonepithelial residue. A tetrahexosylceramide with a terminal Gal alpha 1 leads to 3 on a globotriaosylceramide core was found in both compartments as were homologues with 1 or 2 additional internal leads to 3Gal alpha 1 leads to units, but homologues with 3 or 4 additional internal Gal were only nonepithelial. Glycosphingolipids with terminal beta-GalNAc were restricted to the nonepithelial residue comprising globotetraosylceramide, isoglobotetraosylceramide, and a series of glycolipids with 5 to 9 sugars having the above-mentioned oligohexosylceramides as core structures. Fucolipids (blood group H) having 3, 5, 6, and 7 sugars and lacking amino sugars, and fucolipids with 5 and 10 sugars containing N-acetylglucosamine were restricted to the epithelial cells. Fucolipids (blood groups H and B) with 5 and 6 sugars containing N-acetylgalactosamine were restricted to the nonepithelial residue. In a 4, 6, and 12 sugars were found in the epithelial cells. N-Glycoloylneuraminosyllactosylceramide was the only ganglioside found in the epithelial cells while N-acetylneuraminosyllactosylceramide was nonepithelial together with gangliosides based on gangliotetraosylceramide and isoglobotetraosylceramide.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

Animals

Carbohydrate Conformation

Carbohydrate Sequence

Chromatography

Thin Layer

Epithelium

analysis

Glycosphingolipids

isolation & purification

Ileum

analysis

Intestine

Small

analysis

Jejunum

analysis

Magnetic Resonance Spectroscopy

Mass Spectrometry

Rats

Rats

Inbred Strains

Publikations- och innehållstyp

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