Life-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice

• Whether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germcell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreERT2;R26R and Foxl2-CreERT2;mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Follicle tracing

Genetically modified mouse models

Life-long observations

No postnatal oocyte regeneration

Oocyte tracing

cre recombinase

growth differentiation factor 9

messenger RNA

adult

animal cell

animal experiment

animal tissue

Article

cell lineage

controlled study

corpus luteum

female

gene expression

genetically engineered mouse strain

germline stem cell

lifespan

male

mouse

nonhuman

oocyte development

ovary

primordial germ cell

regeneration

reverse transcription polymerase chain reaction

somatic cell

spermatogenesis

Mus

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