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MiRNA-320 in the hu...
MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro.
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Feng, Ruizhi (författare)
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Sang, Qing (författare)
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Zhu, Yan (författare)
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Fu, Wei (författare)
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Liu, Miao (författare)
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Xu, Yan (författare)
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Shi, Huijuan (författare)
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Xu, Yao (författare)
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Qu, Ronggui (författare)
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Chai, Renjie (författare)
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- Shao, Linus Ruijin, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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Jin, Li (författare)
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He, Lin (författare)
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- Sun, Xiaoxi (författare)
- Karolinska Institutet
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Wang, Lei (författare)
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Shao, RJ (författare)
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(creator_code:org_t)
- 2015-03-03
- 2015
- Engelska.
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Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 5
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https://gup.ub.gu.se... (primary) (free)
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https://doi.org/10.1...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
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- Previous work from our laboratory demonstrated the existence of miRNAs in human follicular fluid. In the current study, we have sought to identify miRNAs that might affect oocyte/embryo quality in patients undergoing intracytoplasmic sperm injection and to investigate their roles in in vitro fertilization outcomes in mouse oocytes. 53 samples were classified as Group 1 (high quality) if the day-3 embryos had seven and more cells or as Group 2 (low quality) if the embryos had six and fewer cells. TaqMan Human microRNAs cards and qRT-PCR were performed to verify differently expressed miRNAs. The function of the corresponding miRNA was investigated in mouse oocytes by injecting them with miRNA-inhibitor oligonucleotides. We found that hsa-miR-320a and hsa-miR-197 had significantly higher expression levels in the Group 1 follicular fluids than in Group 2 (p = 0.0073 and p = 0.008, respectively). Knockdown of mmu-miR-320 in mouse oocytes strongly decreased the proportions of MII oocytes that developed into two-cell and blastocyst stage embryos (p = 0.0048 and p = 0.0069, respectively). Wnt signaling pathway components had abnormal expression level in miR-320 inhibitor-injected oocytes. This study provides the first evidence that miRNAs in human follicular fluid are indicative of and can influence embryo quality.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Feng, Ruizhi
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Sang, Qing
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Zhu, Yan
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Fu, Wei
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Liu, Miao
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Xu, Yan
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visa fler...
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Shi, Huijuan
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Xu, Yao
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Qu, Ronggui
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Chai, Renjie
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Shao, Linus Ruij ...
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Jin, Li
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He, Lin
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Sun, Xiaoxi
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Wang, Lei
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Shao, RJ
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Endokrinologi oc ...
- Artiklar i publikationen
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Scientific repor ...
- Av lärosätet
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Göteborgs universitet
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Karolinska Institutet