Sökning: id:"swepub:oai:gup.ub.gu.se/217391" >
Improved Molecular ...
Improved Molecular Imaging in Rodent Brain with Time-of-Flight-Secondary Ion Mass Spectrometry Using Gas Cluster Ion Beams and Reactive Vapor Exposure
-
- Angerer, Tina B., 1987 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,Departmentof Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden
-
- Dowlatshahi Pour, Masoumeh, 1976 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Departmentof Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden
-
- Malmberg, Per, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Departmentof Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden
-
visa fler...
-
- Fletcher, John S. (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg,Departmentof Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden;Departmentof Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden
-
visa färre...
-
(creator_code:org_t)
- 2015-04-02
- 2015
- Engelska.
-
Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 87:8, s. 4305-4313
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://research.cha...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Imaging mass spectrometry has shown to be a valuable method in medical research and can be performed using different instrumentation and sample preparation methods, each one with specific advantages and drawbacks. Time-of-flight-secondary ion mass spectrometry (TOF-SIMS) has the advantage of high spatial resolution imaging but is often restricted to low mass molecular signals and can be very sensitive to sample preparation artifacts. In this report we demonstrate the advantages of using gas cluster ion beams (GCIBs) in combination with trifluoracetic acid (TFA) vapor exposure for the imaging of lipids in mouse brain sections. There is an optimum exposure to TFA that is beneficial for increasing high mass signal as well as producing signal from previously unobserved species in the mass spectrum. Cholesterol enrichment and crystallization on the sample surface is removed by TFA exposure uncovering a wider range of lipid species in the white matter regions of the tissue, greatly expanding the chemical coverage and the potential application of TOF-SIMS imaging in neurological studies. Ar-4000(+) (40 keV) in combination with TFA treatment facilitates high resolution, high mass imaging closing the gap between TOF-SIMS and matrix-assisted laser desorption ionization (MALDI).
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas