Expression of tight junction proteins and transporters for xenobiotic metabolism at the blood-CSF barrier during development in the nonhuman primate (P. hamadryas)

• The choroid plexus (CP) is rich in barrier mechanisms including transporters and enzymes which can influence drug disposition between blood and brain. We have limited knowledge of their state in fetus. We have studied barrier mechanisms along with metabolism and transporters influencing xenobiotics, using RNAseq and protein analysis, in the CP during the second-half of gestation in a nonhuman primate (Papio hamadryas). There were no differences in the expression of the tight-junctions at the CP suggesting a well-formed fetal blood-CSF barrier during this period of gestation. Further, the fetal CP express many enzymes for phase I-III metabolisms as well as transporters suggesting that it can greatly influence drug disposition and has a significant machinery to deactivate reactive molecules with only minor gestational changes. In summary, the study suggests that from, at least, midgestation, the CP in the nonhuman primate is restrictive and express most known genes associated with barrier function and transport.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Keyword

Choroid plexus

Fetus

Primate

Baboon

Xenobiotic transport

Neuroprotection

Drug metabolism

CEREBROSPINAL FLUID BARRIER

CHOROID-PLEXUS EPITHELIUM

MULTIDRUG-RESISTANCE

DIFFERENTIAL EXPRESSION

BRAIN INTERFACES

KNOCKOUT MICE

RNA-SEQ

RAT

MECHANISMS

PROTECTS

Reproductive Biology

Toxicology

Publication and Content Type

ref (subject category)

art (subject category)