Search: id:"swepub:oai:gup.ub.gu.se/235542" >
Peptide Fragmentati...
Peptide Fragmentation and Surface Structural Analysis by Means of ToF-SIMS Using Large Cluster Ion Sources
-
Yokoyama, Y. (author)
-
Aoyagi, S. (author)
-
Fujii, M. (author)
-
show more...
-
Matsuo, J. (author)
-
- Fletcher, John S. (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
-
Lockyer, N. P. (author)
-
Vickerman, J. C. (author)
-
Passarelli, Melissa, 1983 (author)
-
Havelund, R. (author)
-
Seah, M. P. (author)
-
show less...
-
(creator_code:org_t)
- 2016-03-09
- 2016
- English.
-
In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 88:7, s. 3592-3597
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://research.cha...
-
show less...
Abstract
Subject headings
Close
- Peptide or protein structural analysis is crucial for the evaluation of biochips and biodevices, therefore an analytical technique with the ability to detect and identify protein and peptide species directly from surfaces with high lateral resolution is required. In this report, the efficacy of ToF-SIMS to analyze and identify proteins directly from surfaces is evaluated. Although the physics governing the SIMS bombardment process precludes the ability for researchers to detect intact protein or larger peptides of greater than a few thousand mass unit directly, it is possible to obtain information on the partial structures of peptides or proteins using low energy per atom argon duster ion beams. Large cluster ion beams, such as Ar dusters and C-60 ion beams, produce spectra similar to those generated by tandem MS. The SIMS bombardment process also produces peptide fragment ions not detected by conventional MS/MS techniques. In order to clarify appropriate measurement conditions for peptide structural analysis, peptide fragmentation dependency on the energy of a primary ion beam and ToF-SIMS specific fragment ions are evaluated. It was found that the energy range approximately 6 <= E/n <= 10 eV/atom is most effective for peptide analysis based on peptide fragments and [M + H] ions. We also observed the cleaving of side chain moieties at extremely low-energy E/n <= 4 eV/atom.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- mass-spectrometry
- universal equation
- ar cluster
- argon
- beams
- energy
- yields
- size
- Chemistry
- mass-spectrometry
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Yokoyama, Y.
-
Aoyagi, S.
-
Fujii, M.
-
Matsuo, J.
-
Fletcher, John S ...
-
Lockyer, N. P.
-
show more...
-
Vickerman, J. C.
-
Passarelli, Meli ...
-
Havelund, R.
-
Seah, M. P.
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
-
and Analytical Chemi ...
- Articles in the publication
-
Analytical Chemi ...
- By the university
-
University of Gothenburg
-
Chalmers University of Technology