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Aspects of the quan...
Aspects of the quantitative analysis of neurons in the cerebral cortex.
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- Skoglund, Thomas, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Pascher, Ragnar (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Berthold, Claes-Henric, 1937 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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(creator_code:org_t)
- 1996
- 1996
- Engelska.
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Ingår i: Journal of neuroscience methods. - 0165-0270. ; 70:2, s. 201-10
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- We address three problems concerning the quantitative analysis of nerve cell distribution in the cerebral cortex: (i) preparatory tissue deformation (shrinkage); (ii) difficulties in differentiating between small neurons and astroglia; and (iii) the bias introduced by the counting method. We found that staining with Richardson's solution led to no shrinkage in Vibratome-cut sections of aldehyde-fixed rat brains, but did result in staining of the neurons and left the glial cells unstained. This was in striking contrast to Nissl staining which introduced a linear shrinkage of 20-30% and stained all kinds of cortical cells indiscriminately. A computer-based unbiased counting method was implemented by taking advantage of the stereological procedure referred to as the 'optical disector' (Gundersen, H.J.G. (1986) Stereology of arbitrary particles, J. Microsc., 143: 3-45).
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Animals
- Cell Count
- methods
- Cell Size
- Cerebral Cortex
- cytology
- Image Processing
- Computer-Assisted
- Male
- Neurons
- cytology
- ultrastructure
- Nissl Bodies
- chemistry
- Rats
- Rats
- Sprague-Dawley
- Staining and Labeling
- methods
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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