Expression of vascular endothelial growth factor (VEGF) and its receptors (Flt-1 and Flk-1) following permanent and transient occlusion of the middle cerebral artery in the rat.

• Vascular endothelial growth factor (VEGF) is a known endothelial mitogen and a potent enhancer of vascular permeability although its role in focal cerebral ischemia is still not completely understood. The present report describes the immunohistochemical distribution of VEGF and its 2 receptors, Flt-1 and Flk-1 at day 1 and 3 following permanent and transient middle cerebral artery occlusion (MCAO) in the rat. A bilateral increase in VEGF immunoreactivity, particularly in neurons and blood vessels, was seen in both the experimental designs by day 1. By day 3, the immunoreactivity was restricted chiefly to the lesion side, where reaction was most prominent in the border zones of the infarcts. Immunoreaction to VEGF was more pronounced in cases of permanent MCAO than in transient MCAO. Flt-1 reaction was increased in neurons, glial and endothelial cells after both transient and permanent MCAO. Immunoreactivity to Flk-1 was prominent in glial cells and was present to some extent in endothelial cells. These findings indicate an early upregulation of VEGF and its receptors after permanent as well as transient focal cerebral ischemia in the rat.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Animals

Blood Pressure

Brain Ischemia

metabolism

pathology

physiopathology

Cerebral Arteries

Cerebrovascular Circulation

Endothelial Growth Factors

biosynthesis

genetics

Endothelium

Vascular

metabolism

pathology

Gene Expression Regulation

Ischemic Attack

Transient

metabolism

pathology

physiopathology

Lymphokines

biosynthesis

genetics

Male

Neuroglia

metabolism

pathology

Neurons

metabolism

pathology

Proto-Oncogene Proteins

biosynthesis

genetics

Rats

Rats

Sprague-Dawley

Receptor Protein-Tyrosine Kinases

biosynthesis

genetics

Receptors

Growth Factor

biosynthesis

genetics

Receptors

Vascular Endothelial Growth Factor

Reference Values

Time Factors

Vascular Endothelial Growth Factor A

Vascular Endothelial Growth Factor Receptor-1

Vascular Endothelial Growth Factors

MEDICINE

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)