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Selective mitochond...
Selective mitochondrial DNA degradation following double-strand breaks
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Moretton, A. (författare)
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Morel, F. (författare)
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- Macao, Bertil, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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Lachaume, P. (författare)
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Ishak, L. (författare)
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Lefebvre, M. (författare)
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Garreau-Balandier, I. (författare)
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Vernet, P. (författare)
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- Falkenberg, Maria, 1968 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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Farge, G. (författare)
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(creator_code:org_t)
- 2017-04-28
- 2017
- Engelska.
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Ingår i: Plos One. - : Public Library of Science (PLoS). - 1932-6203. ; 12:4
- Relaterad länk:
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https://journals.plo...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Mitochondrial DNA ( mtDNA) can undergo double-strand breaks (DSBs), caused by defective replication, or by various endogenous or exogenous sources, such as reactive oxygen species, chemotherapeutic agents or ionizing radiations. MtDNA encodes for proteins involved in ATP production, and maintenance of genome integrity following DSBs is thus of crucial importance. However, the mechanisms involved in mtDNA maintenance after DSBs remain unknown. In this study, we investigated the consequences of the production of mtDNA DSBs using a human inducible cell system expressing the restriction enzyme PstI targeted to mitochondria. Using this system, we could not find any support for DSB repair of mtDNA. Instead we observed a loss of the damaged mtDNA molecules and a severe decrease in mtDNA content. We demonstrate that none of the known mitochondrial nucleases are involved in the mtDNA degradation and that the DNA loss is not due to autophagy, mitophagy or apoptosis. Our study suggests that a still uncharacterized pathway for the targeted degradation of damaged mtDNA in a mitophagy/autophagy-independent manner is present in mitochondria, and might provide the main mechanism used by the cells to deal with DSBs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- targeted restriction-endonuclease
- transcription factor-a
- inducible
- expression
- mtdna replication
- repair
- damage
- cells
- recombination
- removal
- disease
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
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Plos One
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Till lärosätets databas
- Av författaren/redakt...
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Moretton, A.
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Morel, F.
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Macao, Bertil, 1 ...
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Lachaume, P.
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Ishak, L.
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Lefebvre, M.
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Garreau-Balandie ...
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Vernet, P.
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Falkenberg, Mari ...
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Farge, G.
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
- Artiklar i publikationen
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Plos One
- Av lärosätet
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Göteborgs universitet