Marked Differences in the Submandibular Salivary Proteome between Sardinian Alcohol-Preferring and Sardinian Alcohol-Non Preferring Rats Revealed by an Integrated Top-Down-Bottom-Up Proteomic Platform

• Sardinian alcohol-preferring (sP) and Sardinian alcohol-non preferring (sNP) rats have been selectively bred for opposite alcohol preference and consumption. Aiming to verify possible differences at the proteomics level between sP and sNP rats, we investigated the salivary proteome by a a liquid chromatography-mass spectrometry top-down-bottom-up integrated approach. For this purpose, submandibular saliva was collected from alcohol-naive sP and sNP rats under isoprenaline stimulation. A total of 200 peptides and proteins were detected and quantified in the two rat lines, 149 of which were characterized in their naturally occurring structure. The data are available via ProteomeXchange with identifier PXD006997. Surprisingly, sP rats exhibited marked quantitative and qualitative differences with respect to sNP rats, namely higher levels of proteoforms originating from submandibular gland protein C, and from submandibular rat protein 2, as well as those of several unidentified peptides and proteins. sP rats expressed some proteins not detectable in sNP rats such as the glutamine and glutamic acid-rich protein (GRP)-CB. The isoform GRP-B, detectable in both rat lines, was more abundant in sNP rats. The submandibular saliva of sNP rats was also characterized by very high levels of GRP-B proteolytic peptides and rat salivary protein 1. Whether these differences could contribute to the opposite alcohol preference and consumption of sP and sNP rats is currently unknown and requires further investigation. © 2017 American Chemical Society.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Nyckelord

GRPs

HPLC-ESI-MS

rat saliva

RSP1

Sardinian alcohol-non preferring rats

Sardinian alcohol-preferring rats

SMGC

SMR2

submandibular gland

top-down-bottom-up proteomics

alcohol

glutamic acid rich protein CB

glutamine

isoprenaline

proteome

rat salivary protein 1

saliva protein

submandibular gland protein C

submandibular rat protein 2

unclassified drug

animal experiment

Article

controlled study

electrospray mass spectrometry

female

liquid chromatography-mass spectrometry

male

nonhuman

priority journal

protein degradation

proteomics

qualitative analysis

rat

rat strain

reversed phase high performance liquid chromatography

saliva

Sardinian alcohol preferring rat

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)