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Bacterial species a...
Bacterial species associated with persistent apical periodontitis exert differential effects on osteogenic differentiation
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Chow, A. T. (författare)
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Quah, S. Y. (författare)
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- Bergenholtz, Gunnar, 1939 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
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Lim, K. C. (författare)
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Yu, V. S. H. (författare)
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Tan, K. S. (författare)
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(creator_code:org_t)
- 2018-08-29
- 2019
- Engelska.
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Ingår i: International Endodontic Journal. - : Wiley. - 0143-2885. ; 52:2, s. 201-210
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- AimTo determine if bacteria associated with persistent apical periodontitis induce species-specific pro-inflammatory cytokine responses in macrophages, and the effects of this species-specific microenvironment on osteogenic differentiation. MethodologyMacrophages were exposed to Enterococcus faecalis, Streptococcus oralis, Streptococcus mitis, Fusobacterium nucleatum, Treponema denticola or Tannerella forsythia, and levels of TNF- and IL-1 elicited were determined by immunoassay. Following treatment of MG-63 pre-osteoblasts with conditioned media from bacteria-exposed macrophages, osteogenic differentiation and viability of osteoblasts were analyzed by Alizarin Red Staining and MTS assay, respectively. Statistical analysis was carried out by one-way anova with the Tukey post-hoc test. Differences were considered to be significant if P<0.05. ResultsMacrophages exposed to Gram-positive bacteria did not produce significant amounts of cytokines. F.nucleatum-challenged macrophages produced up to four-fold more TNF- and IL-1 compared to T.denticola or T.forsythia. Only conditioned media from macrophages treated with Gram-negative bacteria decreased mineralization and viability of osteoblasts. ConclusionsGram-positive bacteria did not impact osteogenic differentiation and appeared innocuous. Gram-negative bacteria, in particular F.nucleatum elicited an enhanced pro-inflammatory response in macrophages, inhibited osteogenic differentiation and reduced cell viability. The findings suggest that the presence of this organism could potentially increase the severity of persistent apical periodontitis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- Fusobacterium nucleatum
- inflammation
- macrophages
- osteoblasts
- persistent apical periodontitis
- necrosis-factor-alpha
- canal-treated teeth
- alkaline-phosphatase
- activity
- root-filled teeth
- bone-formation
- deoxyribonucleic-acid
- periapical lesions
- collagen-synthesis
- interleukin-1-beta
- osteoblast
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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