Analytical Techniques: Shedding Light upon Nanometer-Sized Secretory Vesicles

• Vesicles, small synaptic and large dense core, are the main organelles responsible for cell-to-cell communication. Analysis of nanometer-sized vesicles and accurate assessment of their physiology are major analytical challenges. This review briefly discusses the achievements made in these areas using some of the strongest techniques comprising transmission electron microscopy, stimulated emission depletion microscopy, nanoscale secondary-ion mass spectrometry, and electrochemical cytometry. These techniques provide high spatial and temporal resolution to precisely delve into the structure of these subcellular organelles and shed light on the processes involved in cell signaling. Correlating results of these techniques has furthered our understanding of the relationship between vesicle structure and function. Both the methods and their use in evaluating function are discussed. © 2019 Elsevier Inc.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

electrochemical cytometry

electron microscopy

mass spectrometry

STED

TEM

vesicles

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)