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Colorectal cancer c...
Colorectal cancer cell lines show striking diversity of their O-glycome reflecting the cellular differentiation phenotype
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Madunic, K. (author)
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Zhang, T. (author)
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Mayboroda, O. A. (author)
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Holst, S. (author)
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Stavenhagen, K. (author)
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- Jin, Chunsheng (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Karlsson, Niclas G., 1966 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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Lageveen-Kammeijer, G. S. M. (author)
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Wuhrer, M. (author)
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(creator_code:org_t)
- 2020-03-31
- 2021
- English.
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In: Cellular and Molecular Life Sciences. - : Springer Science and Business Media LLC. - 1420-682X .- 1420-9071. ; 78:1, s. 337-350
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https://doi.org/10.1...
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Abstract
Subject headings
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- Alterations in protein glycosylation in colorectal cancer (CRC) have been extensively studied using cell lines as models. However, little is known about their O-glycome and the differences in glycan biosynthesis in different cell types. To provide a better understanding of the variation in O-glycosylation phenotypes and their association with other molecular features, an in-depth O-glycosylation analysis of 26 different CRC cell lines was performed. The released O-glycans were analysed on porous graphitized carbon nano-liquid chromatography system coupled to a mass spectrometer via electrospray ionization (PGC-nano-LC-ESI-MS/MS) allowing isomeric separation as well as in-depth structural characterization. Associations between the observed glycan phenotypes with previously reported cell line transcriptome signatures were examined by canonical correlation analysis. Striking differences are observed between the O-glycomes of 26 CRC cell lines. Unsupervized principal component analysis reveals a separation between well-differentiated colon-like and undifferentiated cell lines. Colon-like cell lines are characterized by a prevalence of I-branched and sialyl Lewis x/a epitope carrying glycans, while most undifferentiated cell lines show absence of Lewis epitope expression resulting in dominance of truncated alpha 2,6-core sialylated glycans. Moreover, the expression of glycan signatures associates with the expression of glycosyltransferases that are involved in their biosynthesis, providing a deeper insight into the regulation of glycan biosynthesis in different cell types. This untargeted in-depth screening of cell line O-glycomes paves the way for future studies exploring the role of glycosylation in CRC development and drug response leading to discovery of novel targets for the development of anti-cancer antibodies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- O-Glycosylation
- Glycomics
- Cell lines
- Colorectal cancer
- Mass
- spectrometry
- Porous graphitized carbon liquid chromatography
- consensus molecular subtypes
- histo-blood group
- colon-cancer
- selectin
- ligand
- tn antigen
- expression
- glycosylation
- mucin
- carcinoma
- glycans
- Biochemistry & Molecular Biology
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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