Parapseudoflavitalea muciniphila gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a human peritoneal tumour and reclassification of Pseudobacter ginsenosidimutans as Pseudoflavitalea ginsenosidimutans comb. nov

• A Gram-stain-negative, microaerophilic, non-motile, rod-shaped bacterium strain designated PMP191F(T), was isolated from a human peritoneal tumour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism formed a lineage within the family Chitinophagaceae that was distinct from members of the genus Pseudoflavitalea (95.1-95.2% sequence similarity) and Pseudobacter ginsenosidimutans (94.4 % sequence similarity). The average nucleotide identity values between strain PMP191F(T) and Pseudoflavitalea rhizosphaerae T16R-265(T) and Pseudobacter ginsenosidimutans Gsoil 221(T) was 68.9 and 62.3% respectively. The only respiratory quinone of strain PMP191F(T) was MK-7 and the major fatty acids were iso-C-15:0, iso-C-15: 1 G and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c). The polar lipids consisted of phosphatidylethanolamine and some unidenti-fied amino and glycolipids. The G+C content of strain PMP191FT calculated from the genome sequence was 43.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, strain PMP191F(T) represents a novel species and genus for which the name Parapseudoflavitalea muciniphila gen. nov., sp. nov. is proposed. The type strain is PMP191F(T) (=DSM 104999(T)=ATCC BAA-2857(T) = CCUG 72691(T)). The phylogenetic analyses also revealed that Pseudobacter ginsenosidimutans shared over 98 % sequence similarly to members of the genus Pseudoflavitalea. However, the average nucleotide identity value between Pseu-doflavitalea rhizosphaerae T16R-265(T), the type species of the genus and Pseudobacter ginsenosidimutans Gsoil 221(T) was 86.8 %. Therefore, we also propose that Pseudobacter ginsenosidimutans be reclassified as Pseudoflavitalea ginsenosidimutans comb. nov.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

Nyckelord

Pseudomyxoma peritonei

Chitinophagaceae

Parapseudoflavitalea

Pseudoflavitalea

cancer

bacteria

sequences

acid

Microbiology

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