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H-1, C-13 and N-15 ...
H-1, C-13 and N-15 resonance assignment of backbone and IVL-methyl side chain of the S135A mutant NS3pro/NS2B protein of Dengue II virus reveals unique secondary structure features in solution
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- Agback, Peter (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences
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Lesovoy, D. M. (författare)
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- Han, X. (författare)
- Karolinska Institutet
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- Sun, R. H. (författare)
- Karolinska Institutet
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- Sandalova, T. (författare)
- Karolinska Institutet
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- Agback, T. (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences
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- Achour, A. (författare)
- Karolinska Institutet
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- Orekhov, Vladislav, 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
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- 2022-02-12
- 2022
- Engelska.
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Ingår i: Biomolecular Nmr Assignments. - : Springer Science and Business Media LLC. - 1874-2718 .- 1874-270X. ; 16, s. 135-145
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Abstract
Ämnesord
Stäng
- The serotype II Dengue (DENV 2) virus is the most prevalent of all four known serotypes. Herein, we present nearly complete H-1, N-15, and C-13 backbone and H-1, C-13 isoleucine, valine, and leucine methyl resonance assignment of the apo S135A catalytically inactive variant of the DENV 2 protease enzyme folded as a tandem formed between the serine protease domain NS3pro and the cofactor NS2B, as well as the secondary structure prediction of this complex based on the assigned chemical shifts using the TALOS-N software. Our results provide a solid ground for future elucidation of the structure and dynamic of the apo NS3pro/NS2B complex, key for adequate development of inhibitors, and a thorough molecular understanding of their function(s).
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Dengue 2 virus
- NS3 protease
- Flavivirus protease
- NMR chemical shifts
- assignment
- Methyl assignment
- Backbone dynamics
- nmr-spectroscopy
- ns3 protease
- ns2b cofactor
- binding mode
- enhancement
- relaxation
- system
- trosy
- Biophysics
- Spectroscopy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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