Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening

• We report the discovery that in the presence of chaotropic anions (SCN-) the opening of nanometer biological vesicles at an electrified interface often becomes a two-step process (around 30 % doublet peaks). We have then used this to independently count molecules in each subvesicular compartment, the halo and protein dense-core, and the fraction of catecholamine binding to the dense-core is 68 %. Moreover, we differentiated two distinct populations of large dense-core vesicles (LDCVs) and quantified their content, which might correspond to immature (43 %) and mature (30 %) LDCVs, to reveal differences in their biogenesis. We speculate this is caused by an increase in the electrostatic attraction between protonated catecholamine and the negatively charged dense-core following adsorption of SCN-.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

Amperometry

Chaotropic Effect

Hofmeister Series

Large Dense-Core

Vesicles

Vesicle Opening

secretory granule

catecholamine content

vesicular exocytosis

ph

cells

size

transmitters

populations

mechanisms

kinetics

Chemistry

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)