Sökning: id:"swepub:oai:gup.ub.gu.se/314581" >
A Rapid and Cheap M...
A Rapid and Cheap Method for Extracting and Quantifying Lycopene Content in Tomato Sauces: Effects of Lycopene Micellar Delivery on Human Osteoblast-like Cells
-
Mare, R. (författare)
-
Maurotti, S. (författare)
-
Ferro, Yvelise (författare)
-
visa fler...
-
Galluccio, A. (författare)
-
Arturi, Franco (författare)
-
- Romeo, Stefano, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Center for Cardiovascular and Metabolic Research (CMR)
-
Procopio, A. (författare)
-
Musolino, V. (författare)
-
Mollace, V. (författare)
-
Montalcini, T. (författare)
-
Pujia, A. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2022-02-08
- 2022
- Engelska.
-
Ingår i: Nutrients. - : MDPI AG. - 2072-6643. ; 14:3
- Relaterad länk:
-
https://www.mdpi.com...
-
visa fler...
-
https://gup.ub.gu.se...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Identifying and quantifying the beneficial molecules contained in nutraceuticals is essen-tial to predict the effects derived from their consumption. This study explores a cheap and rapid method for quantifying lycopene content from a semi-solid matrix. In addition, it compares the in vitro effects of the extracts obtained from different tomato sauces available on the local market with Osteocol®, a patented tomato sauce from southern Italy. We performed a liquid extraction of lyco-pene using suitable solvents. The lycopene extracted was encapsulated in surfactant micelles and finally tested in vitro on Saos-2 cells. The effects exerted by lycopene on ALP and Wnt/β-catenin pathways were investigated by Western blotting. Hexane was found to be the best solvent for lyco-pene extraction. Spectrophotometrical and HPLC analyses showed similar trends. Osteocol® contained 39 ± 4 mg lycopene per 100 g of sauce, while the best commercial product contained 19 ± 1 mg/100 g. The Osteocol® lycopene extract increased ALP and β-catenin protein expressions in a dose-dependent manner, also showing statistically significant results (p < 0.05 respectively). In con-clusion, despite both techniques showing similar final results, UV/VIS spectrophotometer is prefer-able to HPLC due to its cheap, rapid, and accurate results, as well as for the opportunity to analyze lycopene-loaded micelles. The extraction and release of lycopene to bone cells positively influences the differentiation of osteoblasts and increases the expression of the ALP and β-catenin proteins. As a consequence, as a lycopene-rich sauce, Osteocol® represents a useful supplement in the prevention of osteoporosis compared to its commercial competitors. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Carotenoid
- Lycopene
- Osteoblast
- Osteoporosis
- Tomato sauce
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Mare, R.
-
Maurotti, S.
-
Ferro, Yvelise
-
Galluccio, A.
-
Arturi, Franco
-
Romeo, Stefano, ...
-
visa fler...
-
Procopio, A.
-
Musolino, V.
-
Mollace, V.
-
Montalcini, T.
-
Pujia, A.
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Nutrients
- Av lärosätet
-
Göteborgs universitet